At the behest of a deadly benefactor known only as the Gentleman, the Chameleon must assemble a new Sinister Six: Dr. Octopus, the Vulture, Electro, Mysterio, and the Gentleman's mysterious ward, Pity. But Mysterio, the master of illusion, has a plan of his own! A number of Hollywood people have been targeted for murder, and the Webhead must halt Mysterio's deadly rampage before more lives are claimed.